Laboratory of Structural & Supramolecular Chemistry

Research Projects


References: C.  Structure of macromolecules by X-ray crystallography


  1. “The binding of beta and gamma-cyclodextrins to glycogen phosphorylase b: kinetic and crystallographic studies” N. Pinotsis, D. D. Leonidas, E. D. Chrysina, N. G. Oikonomakos, I. M. Mavridis, Protein Sci. 2003, 12, 1914-1924.

  2. P. Giastas, N. Pinotsis, G. Efthymiou, M. Wilmanns, P. Kyritsis, J- M. Moulis, I. M. Mavridis “The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32 Å resolution. Comparison with other high resolution structures of ferredoxins and contributing structural features to reduction potential values”, J. Biolog. Inorg. Chem. 2006, 11, 445-458.

  3. E. Saridakis, P. Giastas, G. Efthymiou, V. Thoma, J-M. Moulis, P.Kyritsis, I. M. Mavridis "Insight into the protein and solvent contributions to the reduction potentials of [4Fe-4S]2+/+ clusters: Crystal structures of the Allochromatium vinosum ferredoxin variants C57A and V13G and the homologous Escherichia coli ferredoxin" J. Biol. Inorg. Chem. 2009, 14, 783-799.

  4. P. Zou, N. Pinotsis, S. Lange, Y-H. Song, A. Popov, I. Mavridis, O. M. Mayans, M. Gautel, M. Wilmanns “Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk” Nature 2006, 439, 225-228.

  5. N. Pinotsis, S. D. Chatziefthimiou, F. Berkemeier, F. Beuron, I. M. Mavridis, P. V. Konarev, D. I. Svergun, E. Morris, M. Rief, M. Wilmanns "Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties, PLoS Biol. 2012, Feb;10(2):e1001261.

  6. J. R. Birtley, E. Saridakis, E. Stratikos, I. M. Mavridis "Crystal Structure of Human ER Aminopeptidase 2 Reveals Atomic Basis for Distinct Roles in Antigen Processing" Biochemistry 2012, 51, 286–295.

  7. I. Evnouchidou, J. Birtley, S. Seregin, A. Papakyriakou, E. Zervoudi, M. Samiotaki, G. Panayotou, P. Giastas, O. Petrakis, D. Georgiadis, A. Amalfitano,  E. Saridakis, I. M. Mavridis, E. Stratikos “A Common Single Nucleotide Polymorphism in Endoplasmic Reticulum Aminopeptidase 2 Induces a Specificity Switch that Leads to Altered Antigen Processing” J. Immunol. 2012, 189, 2383-2392.

  8. Despoina A.I. Mavridou, Stuart J. Ferguson , Julie M. Stevens  "The interplay between the disulfide bond formation pathway and cytochrome c maturation in Escherichia coli" FEBS Letters  2012, 586, 1702–1707.

  9. D.A.I. Mavridou,       E. Saridakis, P. Kritsiligkou, A.D. Goddard, J.M. Stevens, S.J. Ferguson and C. Redfield. “Oxidation-state-dependent protein-protein interactions in disulfide cascades.” J. Biol. Chem. 2011, 286, 24943-24956.

  10. D.A.I. Mavridou,       E. Saridakis, P. Kritsiligkou, E.C. Mozley, S.J. Ferguson and C. Redfield. “An extended active-site motif controls the reactivity of the thioredoxin fold.” 2013, submitted to J. Biol. Chem

  11. A. Boudjemline, E. Saridakis, M. J. Swann, L. Govada, I. M. Mavridis, N. E. Chayen "Use of Dual Polarisation Interferometry as a diagnostic tool for protein crystallisation", Anal. Chem. 2011, 83, 7881-7887.

  12. E. Saridakis. “A novel Genetic Algorithm-inspired concept for macromolecular crystal optimisation.” Crystal Growth Des. (2011) 11, 2993-2998

  13. Naomi E. Chayen & Emmanuel Saridakis “Protein crystallization: from purified protein to diffraction-quality crystal.” Nature Methods 2008, 5, 147-153.

  14. Emmanuel Saridakis & Naomi E. Chayen “Towards a ‘universal’ nucleant for protein crystallization.” Trends Biotechnol. 2009, 27, 99-106.

  15. P. Asanithi, E. Saridakis, L. Govada, I. Jurewicz, E.W. Brunner, R. Ponnusamy, J.A.S. Cleaver,  A.B. Dalton, N.E. Chayen & R.P. Sear “Carbon-nanotube based materials for protein crystallisation.” ACS Appl. Mat. Interf. 2009, 1, 1203-1210.

  16. E. Saridakis, S. Khurshid, L. Govada, Q. Phan, D. Hawkins, G.V. Crichlow, E. Lolis, S.M. Reddy and N.E. Chayen. “Protein crystallization facilitated by molecularly imprinted polymers.” Proc. Natl. Acad. Sci. USA 2011, 108, 11081-11086.

  17. E. Zervoudi, E. Saridakis, J.R. Birtley, S. Seregin , E. Reeves, P. Kokkala, Y.A. Aldhamen, A. Amalfitano, I.M. Mavridis, E. James, D. Georgiadis and E. Stratikos (2013) “A rationally designed inhibitor targeting antigen-trimming aminopeptidases enhances antigen presentation and cytotoxic T-cell responses.” Proc. Natl. Acad. Sci. USA 2013, 110, 19890-19895.


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