Laboratory of Structural & Supramolecular Chemistry

Dr. Emmanuel Saridakis

 

Emmanuel Saridakis obtained a BSc degree in Physics in 1991 and a PhD in Biophysics in 1995 at the Physics Department of Imperial College London. His research topic, under the supervision of Professor David Blow, was the study of nucleation and growth of protein crystals. He also holds a University of London postgraduate degree in the History and Philosophy of Science. He has worked as a research associate at the Physics Department and the Faculty of Medicine of Imperial College London, at the University of Athens and at the Institute of Molecular Biology and Biotechnology/ FORTH in Heraklion, Crete.  He joined the Laboratory of Structural and Supramolecular Chemistry at the Institute of Physical Chemistry of NCSR “Demokritos” in 2006 and became a Researcher D in 2008 and Researcher C in 2012. He is also an Honorary Research Fellow at the Imperial College, Faculty of Medicine. His research interests are in the theory and methodology of macromolecular crystallization and in macromolecular crystallography.

 

 Publications

1.       Emmanuel E.G. Saridakis, Patrick D. Shaw Stewart, Lesley F. Lloyd and David M. Blow. “Phase diagram and dilution experiments in the crystallization of carboxypeptidase G2.” Acta Crystallogr. (1994) D50, 293-297.

2.  David M. Blow, Naomi E. Chayen, Lesley F. Lloyd and Emmanuel Saridakis. “Control of nucleation of protein crystals.” Protein Science (1994) 3, 1638-1643.

3.  Naomi E. Chayen, Elspeth J. Gordon, Simon E.V. Phillips, Emmanuel E.G. Saridakis and Peter F. Zagalsky. “Crystallization and initial X-ray analysis of β-crustacyanin, the dimer of apoproteins A2 and C1, each with a bound astaxanthin molecule.” Acta Crystallogr. (1996) D52, 409-410.

4.  Emmanuel Saridakis. “Optimization of the critical nuclear size for protein crystallization: a note.” Acta Crystallogr. (2000) D56, 106-108.

5.  Emmanuel Saridakis and Naomi E. Chayen. “Improving protein crystal quality by decoupling nucleation and growth in vapor diffusion.” Protein Science (2000) 9, 755-757.

6.  Naomi E. Chayen and Emmanuel Saridakis. “Is lysozyme really the ideal model protein?” J. Crystal Growth (2001) 232, 262-264.

7.  N. E. Chayen, E. Saridakis, R. El-Bahar and Y.Nemirovsky. “Porous Silicon: an Effective Nucleation-inducing Material for Protein Crystallization.” J.Mol.Biol. (2001) 312, 591-595.

8.  Abel Moreno, Emmanuel Saridakis and Naomi E. Chayen. “Combinations of oils and gels for enhancing the growth of protein crystals.” J.Appl.Cryst. (2002) 35, 140-142.

9.  Naomi E. Chayen and Emmanuel Saridakis. “Protein crystallization for genomics: towards high throughput optimisation techniques.” (lead article) Acta Crystallogr. (2002) D58, 921-927.  

10. Emmanuel Saridakis, Abel Moreno, Karsten Dierks, Matthias Dieckmann and Naomi Chayen. “Separating nucleation and growth in protein crystallization using Dynamic Light Scattering.” Acta Crystallogr. (2002) D58, 1597-1600.  

11. Anita Penkova, Naomi E. Chayen, Emmanuel Saridakis and Christo Nanev. “Nucleation of protein crystals in a wide continuous supersaturation gradient.” Acta Crystallogr. (2002) D58, 1606-1610.  

12. Emmanuel Saridakis and Naomi E. Chayen. “Systematic Improvement of Protein Crystals by Determining the Supersolubility Curves of Phase Diagrams.” Biophys. J. (2003) 84, 1218-1222.  

13. Richard Sears, Emmanuel Saridakis and Naomi E. Chayen. “Experiment and theory for heterogeneous nucleation of protein crystals in a porous medium.” Proc. Natl. Acad. Sci. U.S.A. (2006) 103, 597-601.  

14. S. Stolyarova, E. Saridakis, N.E. Chayen & Y. Nemirovsky. “A Model for Enhanced Nucleation of Protein Crystals on a Fractal Porous Substrate.” Biophys. J. (2006) 91, 3857-3863.  

15. Naomi E. Chayen and Emmanuel Saridakis. “Protein crystallization: from purified protein to diffraction-quality crystal.” Nature Methods (2008) 5, 147-153.  

16. Emmanuel. Saridakis and Naomi E. Chayen. “Towards a ‘universal’ nucleant for protein crystallization.” Trends Biotechnol.  (2009) 27, 99-106  

17. E. Saridakis, P. Giastas, G. Efhtymiou, V. Thoma, J.-M. Moulis, P. Kyritsis & I.M. Mavridis. “Insight into the protein and solvent contributions to the reduction potentials of [4Fe–4S]2+/+ clusters: crystal structures of the Allochromatium vinosum ferredoxin variants C57A and V13G and the homologous Escherichia coli ferredoxin.” J. Biol. Inorg. Chem. (2009) 14, 783-799.

18. Piyapong Asanithi, Emmanuel Saridakis, Lata Govada, Izabela Jurewicz, Eric W. Brunner, Rajesh Ponnusamy, Jamie A.S. Cleaver,  Alan B. Dalton REF _RefF1 \h  \* MERGEFORMAT , Naomi E. Chayen & Richard P. Sear. “Carbon-nanotube based materials for protein crystallisation.” ACS Appl. Mat. Interf. (2009) 1, 1203-1210.

19. A. Kaloudi-Chantzea, N. Karakostas, C.P. Raptopoulou, V. Psycharis, E. Saridakis, J. Griebel, R. Hermann and G. Pistolis. “Coordination-driven self assembly of a brilliantly fluorescent rhomboid cavitand composed of bodipy-dye subunits.” J. Am. Chem. Soc. (2010) 132, 16327-16329.

20. A. Garcia-Caballero, J.A. Gavira, E. Pineda-Molina, N.E. Chayen, L. Govada, S. Khurshid, E. Saridakis, A. Boudjemline, M.J. Swann, P. Shaw Stewart, R.A. Briggs, S.A. Kolek, D. Oberthuer, K. Dierks, C. Betzel, M. Santana, J.R. Hobbs, P. Thaw, T.J. Savill,  J.R. Mesters, R. Hilgenfeld, N. Bonander and R.M. Bill. “Optimization of Protein Crystallization: The OptiCryst Project.” Cryst. Growth Des. (2011) 11, 2112–2121.

21. D.A.I. Mavridou, E. Saridakis, P. Kritsiligkou, A.D. Goddard, J.M. Stevens, S.J. Ferguson and C. Redfield. “Oxidation-state-dependent protein-protein interactions in disulfide cascades.” J. Biol. Chem. (2011) 286, 24943-24956.

22. I.A. Katsoulis, G. Kythreoti, A. Papakyriakou, K. Koltsida, P. Anastasopoulou, C.I. Stathakis, I. Mavridis, T. Cottin, E. Saridakis and D. Vourloumis. “Synthesis of 5,6-spiroethers and evaluation of their affinities for the bacterial A-site.” ChemBioChem (2011) 12, 1188-1192.

23. E. Saridakis. “A novel Genetic Algorithm-inspired concept for macromolecular crystal optimisation.” Crystal Growth Des. (2011) 11, 2993-2998.

24.    E. Saridakis, S. Khurshid, L. Govada, Q. Phan, D. Hawkins, G.V. Crichlow, E. Lolis, S.M. Reddy and N.E. Chayen. “Protein crystallization facilitated by molecularly imprinted polymers.” Proc. Natl. Acad. Sci. USA (2011) 108, 11081-11086.

25.    A. Boudjemline, E. Saridakis, M.J. Swann, L. Govada, I.M. Mavridis and Chayen, N.E. “Use of Dual Polarisation Interferometry as a diagnostic tool for protein crystallisation.” Anal. Chem. (2011) 83, 7881-7887.

26.    J. Birtley, E. Saridakis, E. Stratikos and I.M. Mavridis “Crystal structure of human ER Aminopeptidase 2 reveals atomic basis for distinct roles in antigen processing.” Biochemistry (2012) 51, 286-295.

27.    E. Saridakis “Perspectives on high-throughput technologies applied to protein crystallisation.” Protein Pept. Lett.  (2012) 19, 778-783.

28.    I. Evnouchidou, J. Birtley, S. Seregin, A. Papakyriakou, E. Zervoudi, M. Samiotaki, G. Panayotou, P. Giastas, O. Petrakis, D. Georgiadis, A. Amalfitano, E. Saridakis, I.M. Mavridis, and E. Stratikos. “A common SNP in ER aminopeptidase 2 induces a specificity switch that leads to altered antigen processing.” J. Immunol. (2012) 189, 2383-2392.

29.    Emmanuel Saridakis and Naomi E. Chayen. “Imprinted polymers assisting protein crystallization.” Trends Biotechnol. (2013) 31, 515-520.

30.    E. Zervoudi, E. Saridakis, J.R. Birtley, S. Seregin , E. Reeves, P. Kokkala, Y.A. Aldhamen, A. Amalfitano, I.M. Mavridis, E. James, D. Georgiadis and E. Stratikos (2013) “A rationally designed inhibitor targeting antigen-trimming aminopeptidases enhances antigen presentation and cytotoxic T-cell responses.” Proc. Natl. Acad. Sci. USA (2013) in press.

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